Dataset Information

Ocular and Serum Profiles of Inflammatory Molecules Associated With Retinitis Pigmentosa.

ABSTRACT:

Purpose

To investigate the profiles and correlations between local and systemic inflammatory molecules in patients with retinitis pigmentosa (RP).

Methods

The paired samples of aqueous humor and serum were collected from 36 eyes of 36 typical patients with RP and 25 eyes of age-matched patients with cataracts. The concentration of cytokines/chemokines was evaluated by a multiplexed immunoarray (Q-Plex). The correlations between ocular and serum inflammatory molecules and their association with visual function were analyzed.

Results

The aqueous levels of IL-6, Eotaxin, GROα, I-309, IL-8, IP-10, MCP-1, MCP-2, RANTES, and TARC were significantly elevated in patients with RP compared to controls (all P < 0.05). The detection rate of aqueous IL-23 was higher in patients with RP (27.8%) compared with controls (0%). In patients with RP, Spearman correlation test demonstrated positive correlations for IL-23, I-309, IL-8, and RANTES between aqueous and serum expression levels (IL-23: ⍴ = 0.8604, P < 0.0001; I-309: ρ = 0.4172, P = 0.0113; IL-8: ρ = 0.3325, P = 0.0476; RANTES: ρ = 0.6685, P < 0.0001). In addition, higher aqueous IL-23 was associated with faster visual acuity loss in 10 patients with RP with detected aqueous IL-23 (ρ = 0.4119 and P = 0.0264). Multiple factor analysis confirmed that aqueous and serum IL-23 were associated with visual acuity loss in patients with RP.

Conclusions

These findings suggest that ocular and systemic inflammatory responses have a close interaction in patients with RP. Further longitudinal studies with larger cohorts are needed to explore the correlation between specific inflammatory pathways and the progression of RP.

Translational relevance

This study demonstrates the local-systemic interaction of immune responses in patients with RP.

SUBMITTER: Tao Y

PROVIDER: S-EPMC11318359 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature

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Publications

Ocular and Serum Profiles of Inflammatory Molecules Associated With Retinitis Pigmentosa.

Tao Yan Y Fukushima Masatoshi M Shimokawa Sakurako S Zhao Huanyu H Okita Ayako A Fujiwara Kohta K Takeda Atsunobu A Mukai Shizuo S Sonoda Koh-Hei KH Murakami Yusuke Y

Translational vision science & technology 20240801 8

<h4>Purpose</h4>To investigate the profiles and correlations between local and systemic inflammatory molecules in patients with retinitis pigmentosa (RP).<h4>Methods</h4>The paired samples of aqueous humor and serum were collected from 36 eyes of 36 typical patients with RP and 25 eyes of age-matched patients with cataracts. The concentration of cytokines/chemokines was evaluated by a multiplexed immunoarray (Q-Plex). The correlations between ocular and serum inflammatory molecules and their ass ...[more]

PMID: 39120884

Similar Datasets

Project description:PurposeRetinal degeneration involves neuroinflammation, and pro-inflammatory cytokines/chemokines are markedly increased in the eyes of patients with retinitis pigmentosa (RP). In this study, we investigated the changes of serum cytokines/chemokines in RP, and their relationships with visual parameters.MethodsForty-five consecutive patients with typical RP aged 20 to -39 years and 28 age-matched and gender-matched controls were included. Fifteen cytokines (interleukin [IL]-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, 1L-15, IL-17, IL-23, interferon [IFN]-γ, and tumor necrosis factor [TNF]-α, TNF-β) and 9 chemokines (eotaxin, growth-related oncogene [GRO]-α, I-309, IL-8, IFN-γ-inducible protein [IP]-10, monocyte chemotactic protein [MCP]-1, MCP-2, regulated activation normal T-cell expressed and secreted [RANTES], and thymus and activated regulated chemokine [TARC]) in the serum were simultaneously measured by a multiplexed immunoarray (Q-Plex). Relationships between these cytokines/chemokines and indices of central vision, such as visual acuity (VA), the values of static perimetry tests (Humphrey Field analyzer, the central 10-2 program), and optical coherence tomography measures were analyzed in the patients with RP.ResultsAmong the 15 cytokines and 9 chemokines, serum IL-8 and RANTES levels were significantly increased in patients with RP compared with controls (IL-8: P < 0.0001; RANTES: P < 0.0001). Among the elevated cytokines/chemokines, the levels of IL-8 were negatively correlated with VA (ρ = 0.3596 and P = 0.0165), and the average retinal sensitivity of four central points (ρ = -0.3691 and P = 0.0291), and 12 central points (ρ = -0.3491 and P = 0.0398), as well as the central subfield thickness (ρ = -0.3961 and P = 0.0094), and ellipsoid zone width (ρ = -0.3841 and P = 0.0120).ConclusionsPeripheral inflammatory response may be activated and serum IL-8 levels are associated with central vision in patients with RP.

Dataset Information

Ocular and Serum Profiles of Inflammatory Molecules Associated With Retinitis Pigmentosa.

Purpose

Methods

Results

Conclusions

Translational relevance

Publications

Ocular and Serum Profiles of Inflammatory Molecules Associated With Retinitis Pigmentosa.

Similar Datasets

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