Practical PCR genotyping protocols for Plasmodium vivax using Pvcs and Pvmsp1.

ABSTRACT:

Background

Plasmodium vivax is the second most prevalent malaria parasite affecting more than 75 million people each year, mostly in South America and Asia. In addition to major morbidity this parasite is associated with relapses and a reduction in birthweight. The emergence and spread of drug resistance in Plasmodium falciparum is a major factor in the resurgence of this parasite. P. vivax resistance to drugs has more recently emerged and monitoring the situation would be helped, as for P. falciparum, by molecular methods that can be used to characterize parasites in field studies and drug efficacy trials.

Methods

Practical PCR genotyping protocols based on polymorphic loci present in two P. vivax genetic markers, Pvcs and Pvmsp1, were developed. The methodology was evaluat

SUBMITTER: Imwong M 

PROVIDER: S-EPMC1131918 | biostudies-literature | 2005 Apr

REPOSITORIES: biostudies-literature