CTHRC1 modulates cell proliferation and invasion in hepatocellular carcinoma by DNA methylation.

ABSTRACT:

Background

Collagen triple helix repeat containing-1 (CTHRC1), an extracellular matrix protein, is highly expressed in hepatocellular carcinoma (HCC) and linked to poor prognosis. Nevertheless, the precise mechanism of CTHRC1 in HCC is unclear.

Methods

Agena MassARRAY® Methylation Analysis assessed the methylation level of CTHRC1 in the promoter region. Functional assays were conducted to investigate the effects of CTHRC1 knockdown in Hep3B2.1 cells. RNA sequencing identified differentially expressed genes and lncRNAs associated with angiogenesis after CTHRC1 knockdown. Furthermore, differential alternative splicing (AS) and gene fusion events were analyzed using rMATS and Arriba.

Results

In HCC cell lines, CTHRC1 was highly expressed and associated with hypomethylati

SUBMITTER: Sun X 

PROVIDER: S-EPMC11319694 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature