Candidate proteins interacting with cytoskeleton in cells from the basal airway epithelium in vitro.

ABSTRACT: Introduction: The cytoskeleton consists of actin, microtubules, septins, and intermediate filaments and, in most cells, is anchored to an extracellular matrix. Each cell has a unique arrangement of this network and readjusts it from time to time. To investigate the regulation of these reorganizations, we identified interactors from extracts of four cultured lines representing basal cells from the airway epithelium. Methods: After immunoprecipitation with an antibody against keratin 17, samples were processed by liquid chromatography and tandem mass spectrometry. Samples not undergoing antibody-mediated capture were processed in parallel. Results: The main keratins of basal cells, namely, Krt14 (type I) and Krt5 (type II), constituted 67% of the total keratin recovered.

SUBMITTER: Olatona OA 

PROVIDER: S-EPMC11319710 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature