Dataset Information

Antiseptics' Concentration, Combination, and Exposure Time on Bacterial and Fungal Biofilm Eradication.

ABSTRACT:

Background

This study aims to assess the activity of solutions containing povidone-iodine (PI) and hydrogen peroxide (H₂O₂) alone or combined on the biofilm of microbial species in the contest of periprosthetic joint infection (PJI).

Methods

Different antiseptic solutions were tested on 2-day-old biofilms of Gram-positive and Gram-negative bacteria and fungi at 1 and 3 minutes of exposure. The efficacy of these solutions was evaluated by measuring the biofilm metabolic activity by methoxynitrosulfophenyl-tetrazolium carboxanilide (XTT) reduction assay. The anti-biofilm effect of 5% PI and 0.3% PI + 0.5% H₂O₂ was tested on a 5-day-old biofilm using colony-forming unit counts and an XTT reduction assay.

Results

PI and H₂O₂ solutions showed concentration-dependent anti-biofilm activity except for E. faecalis. PI at 5% was the most active solution against the 2-day-old biofilm of all test microorganisms. The 0.3% PI + 0.5% H₂O₂ solution had a significant effect only at 3 minutes. The 5% PI and 0.3% PI + 0.5% H₂O₂ effect was evaluated on 5-day-old biofilms. PI at 5% produced a significant reduction in metabolic activity at both 1 and 3 minutes; 0.3% PI + 0.5% H₂O₂ caused a significant activity against all Gram-positive strains after 3 minutes, with a greater metabolic activity reduction than 5% PI.

Conclusions

In the case of PJI caused by Gram-positive bacteria, 0.3% PI + 0.5% H₂O₂ could be used for wound irrigation for 3 minutes of exposure. In the case of PJI with a different etiological agent or PJI with an unknown etiology, it is advisable to use 5% PI for 1 minute of exposure.

SUBMITTER: Roscetto E

PROVIDER: S-EPMC11320471 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature

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Publications

Antiseptics' Concentration, Combination, and Exposure Time on Bacterial and Fungal Biofilm Eradication.

Roscetto Emanuela E Di Gennaro Donato D Ascione Tiziana T Galdiero Umberto U Aversa Martina M Festa Enrico E Catania Maria Rosaria MR Balato Giovanni G

Arthroplasty today 20240723

<h4>Background</h4>This study aims to assess the activity of solutions containing povidone-iodine (PI) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) alone or combined on the biofilm of microbial species in the contest of periprosthetic joint infection (PJI).<h4>Methods</h4>Different antiseptic solutions were tested on 2-day-old biofilms of Gram-positive and Gram-negative bacteria and fungi at 1 and 3 minutes of exposure. The efficacy of these solutions was evaluated by measuring the biofilm ...[more]

PMID: 39139360

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Dataset Information

Antiseptics' Concentration, Combination, and Exposure Time on Bacterial and Fungal Biofilm Eradication.

Background

Methods

Results

Conclusions

Publications

Antiseptics' Concentration, Combination, and Exposure Time on Bacterial and Fungal Biofilm Eradication.

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