Galectin-3 induces pathogenic immunosuppressive macrophages through interaction with TREM2 in lung cancer.

ABSTRACT:

Background

High infiltration of tumor-associated macrophages (TAMs) is associated with tumor promotion and immunosuppression. The triggering receptor expressed on myeloid cells 2 (TREM2) is emerged as a key immunosuppressive regulator for TAMs, however, how TREM2-expressing TAMs are recruited and what ligands TREM2 interacts with to mediate immunosuppression is unknown.

Methods

Flow cytometry and single-cell RNA sequencing were used to analyze TREM2 expression. Mechanistically, mass spectrometry and immunoprecipitation were employed to identify proteins binding to TREM2. Phagocytosis and co-culture experiments were used to explore the in vitro functions of galectin3-TREM2 pair. Establishment of TREM2^f/f-Lyz2-cre mice to validate the role of TREM2 signaling pathway

SUBMITTER: Wang Q 

PROVIDER: S-EPMC11321020 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature