LRRC56 is an IFT cargo required for assembly of the distal dynein docking complex in Trypanosoma brucei.

ABSTRACT: Outer dynein arms (ODAs) are responsible for ciliary beating in eukaryotes. They are assembled in the cytoplasm and shipped by intraflagellar transport (IFT) before attachment to microtubule doublets via the docking complex. The LRRC56 protein has been proposed to contribute to ODAs maturation. Mutations or deletion of the LRRC56 gene lead to reduced ciliary motility in all species investigated so far, but with variable impact on dynein arm presence. Here, we investigated the role of LRRC56 in the protist Trypanosoma brucei, where its absence results in distal loss of ODAs, mostly in growing flagella. We show that LRRC56 is a transient cargo of IFT trains during flagellum construction and surprisingly, is required for efficient attachment of a subset of docking complex protei

SUBMITTER: Bonnefoy S 

PROVIDER: S-EPMC11321045 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature