Anti-signal recognition particle antibodies induce cardiac diastolic dysfunction via oxidative stress injury.

ABSTRACT:

Objectives

Anti-signal recognition particle (SRP) antibodies, markers of immune-mediated necrotising myopathy, are reportedly related to cardiac involvement; however, whether they are pathogenic to the myocardium remains unclear. We aimed, therefore, to explore the pathogenicity of anti-SRP antibodies against the myocardium through in vivo and in vitro studies.

Methods

Total immunoglobulin G (IgG), purified from patients with positive anti-SRP antibodies, was passively transferred into C57BL/6 mice. Cardiac function was evaluated via echocardiography and the ventricular pressure-volume loop; cardiac histological changes were analysed using haematoxylin-eosin staining, picrosirius red staining, immunofluorescence and immunohistochemistry. Additionally, reactive

SUBMITTER: Zhang H 

PROVIDER: S-EPMC11321054 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature