Orai1 and Orai3 act through distinct signalling axes to promote stemness and tumorigenicity of breast cancer stem cells.

ABSTRACT:

Background

One of major challenges in breast tumor therapy is the existence of breast cancer stem cells (BCSCs). BCSCs are a small subpopulation of tumor cells that exhibit characteristics of stem cells. BCSCs are responsible for progression, recurrence, chemoresistance and metastasis of breast cancer. Ca²⁺ signalling plays an important role in diverse processes in cancer development. However, the role of Ca²⁺ signalling in BCSCs is still poorly understood.

Methods

A highly effective 3D soft fibrin gel system was used to enrich BCSC-like cells from ER+ breast cancer lines MCF7 and MDA-MB-415. We then investigated the role of two Ca²⁺-permeable ion channels Orai1 and Orai3 in the growth and stemness of BCSC-like cells in vitro, and tumorigenic

SUBMITTER: Zhuo D 

PROVIDER: S-EPMC11321067 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature