MiR-876-3p is a tumor suppressor on 9p21 that is inactivated in melanoma and targets ERK.

ABSTRACT:

Background

While melanomas commonly harbor losses of 9p21, on which CDKN2A resides, the presence of additional tumor suppressor elements at this locus is incompletely characterized. Here we assess the expression levels and functional role of microRNA-876-3p (miR-876), whose gene also maps to 9p21.

Methods

Expression of miR-876 was assessed in human tissues and cell lines using quantitative miRNA reverse transcriptase polymerase chain reaction (qRT-PCR). MIR876 copy number was determined in The Cancer Genome Atlas (TCGA) melanoma cohort. The consequences of regulation of miR-876 expression were assessed on melanoma cell colony formation, migration, invasion, apoptosis, cell cycle progression, and drug sensitivity in culture, and on in vivo tumor growth in a xenograft model. G

SUBMITTER: Bezrookove V 

PROVIDER: S-EPMC11321151 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature