Effect of cryopreservation and semen extender on extracellular vesicles isolated from bull semen.

ABSTRACT:

Introduction

Semen cryopreservation is the most popular practice for semen production for artificial insemination and in vitro fertilization in cattle. The Seminal plasma contains extracellular vesicles (spEVs) which modulate sperm viability and function during oocyte fecundation. The study of spEVs in frozen-thawed semen doses may yield novel indicators for predicting bull fertility, but the presence of the semen extender may hinder molecular profiling of spEVs. The aim of this study was to provide extensive characterization of EVs isolated from seminal plasma before and after the cryopreservation process and the addition of a commercial animal protein-free semen extender to understand the potential influence of EVs originating from the extender in hindering the use of spEV

SUBMITTER: Capra E 

PROVIDER: S-EPMC11321215 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature