Quantification of low-level human cytomegalovirus and Epstein-Barr virus DNAemia by digital PCR.

ABSTRACT:

Background

Digital PCR (dPCR) can quantify cell-free viral DNA (DNAemia), a biomarker of active viral infection. To accelerate epidemiologic investigation into low-level viral reactivation in chronic disease, we have evaluated the performance of dPCR to detect cytomegalovirus (CMV) and Epstein-Barr virus (EBV) DNAemia across platforms and blood matrices.

Methods

The droplet-based (BioRad) dPCR platform performance was compared to chip-based (BioMark), and assay validation followed dMIQE guidelines. CMV and EBV DNA reference materials were spiked into known negative plasma and serum samples. In addition, two independent cohorts of ovarian cancer patients were evaluated for viral DNAemia (n = 65 serum and 79 plasma samples).

Results

The limit of quantification (LOQ) was

SUBMITTER: Hunter-Schlichting DN 

PROVIDER: S-EPMC11321275 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature