Identification and biophysical characterization of a novel domain-swapped camelid antibody specific for fentanyl.

ABSTRACT: Opioid use disorders (OUD) and overdoses are ever-evolving public health threats that continue to grow in incidence and prevalence in the United States and abroad. Current treatments consist of opioid receptor agonists and antagonists, which are safe and effective but still suffer from some limitations. Murine and humanized monoclonal antibodies (mAb) have emerged as an alternative and complementary strategy to reverse and prevent opioid-induced respiratory depression. To explore antibody applications beyond traditional heavy-light chain mAbs, we identified and biophysically characterized a novel single-domain antibody specific for fentanyl from a camelid variable-heavy-heavy (VHH) domain phage display library. Structural data suggested that VHH binding to fentanyl was facilitated by a uni

SUBMITTER: Gallant JP 

PROVIDER: S-EPMC11321312 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature