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Modular polyketide synthase ketosynthases collaborate with upstream dehydratases to install double bonds.

ABSTRACT: A VMYH motif was determined to help ketosynthases in polyketide assembly lines select α,β-unsaturated intermediates from an equilibrium mediated by an upstream dehydratase. Alterations of this motif decreased ketosynthase selectivity within a model tetraketide synthase, most significantly when replaced by the TNGQ motif of ketosynthases that accept D-β-hydroxy intermediates.

SUBMITTER: Ray KA

PROVIDER: S-EPMC11321453 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature

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Modular polyketide synthase ketosynthases collaborate with upstream dehydratases to install double bonds.

Ray Katherine A KA Saif Nisha N Keatinge-Clay Adrian T AT

Chemical communications (Cambridge, England) 20240813 66

A VMYH motif was determined to help ketosynthases in polyketide assembly lines select α,β-unsaturated intermediates from an equilibrium mediated by an upstream dehydratase. Alterations of this motif decreased ketosynthase selectivity within a model tetraketide synthase, most significantly when replaced by the TNGQ motif of ketosynthases that accept D-β-hydroxy intermediates. ...[more]

PMID: 39056119

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