Astaxanthin Alleviates Autoimmune Hepatitis by Modulating CD8⁺ T Cells: Insights From Mass Cytometry and Single-Cell RNA Sequencing Analyses.

ABSTRACT: Astaxanthin (ASX) is an oxygen-containing non-vitamin A carotenoid pigment. However, the role of ASX in autoimmune hepatitis (AIH) remains unclear. In this study, a mouse model of AIH is established induced by concanavalin A (ConA). Mass cytometry and single-cell RNA sequencing (scRNA-seq) are used to analyze the potential role of ASX in regulating the immune microenvironment of AIH. ASX treatment effectively alleviated liver damage induced by ConA and downregulated pro-inflammatory cytokines production in mice. Mass cytometry and scRNA-seq analyses revealed a significant increase in the number of CD8⁺ T cells following ASX treatment. Functional markers of CD8⁺ T cells, such as CD69, MHC II, and PD-1, are significantly downregulated. Additionally, specific CD8^+</

SUBMITTER: He Y 

PROVIDER: S-EPMC11321693 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature