Robust detection of clinically relevant features in single-cell RNA profiles of patient-matched fresh and formalin-fixed paraffin-embedded (FFPE) lung cancer tissue.

ABSTRACT:

Purpose

Single-cell transcriptional profiling reveals cell heterogeneity and clinically relevant traits in intra-operatively collected patient-derived tissue. So far, single-cell studies have been constrained by the requirement for prospectively collected fresh or cryopreserved tissue. This limitation might be overcome by recent technical developments enabling single-cell analysis of FFPE tissue.

Methods

We benchmark single-cell profiles from patient-matched fresh, cryopreserved and archival FFPE cancer tissue.

Results

We find that fresh tissue and FFPE routine blocks can be employed for the robust detection of clinically relevant traits on the single-cell level. Specifically, single-cell maps of fresh patient tissues and corresponding FFPE tissue blocks could be inte

SUBMITTER: Trinks A 

PROVIDER: S-EPMC11322409 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature