Evaluation of a multiplex liquid chromatography-tandem mass spectrometry method for congenital adrenal hyperplasia in pediatric patients.

ABSTRACT: Multiplexed adrenal steroid measurement provides critical diagnostic information for patients with congenital adrenal hyperplasia (CAH) as confirmation of newborn screening (NBS) or as initial diagnosis. This study reports the implementation of an adrenal steroid profiling method with a turnaround time (TAT) of less than 24 h using liquid chromatography and tandem-mass spectrometry (LC-MS/MS). A lab-developed multiplexed LC-MS/MS assay was used to quantify levels of 11-deoxycortisol, cortisol, 17-hydroxy-progesterone (17-OHP), androstenedione, and testosterone. Intra and interassay imprecision were found to be <10%. Comparison with a reference laboratory revealed <20% bias for all 5 analytes and Deming correlation coefficients >0.990. Linearity ranges were established from the lowest to up

SUBMITTER: Cao J 

PROVIDER: S-EPMC11322746 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature