Multiplexed analysis of steroid hormones in saliva by LC-MS/MS with 2-hydrazinopyridine derivatization.

ABSTRACT:

Background

LC-MS/MS methods for multiplexed analysis of steroid hormones in saliva are useful research tools in endocrinology, but require sensitive detection.

Objective

To explore the use of hydrazide and hydrazine derivatization to improve sensitivity of detection for ketosteroids. On development and validation of a sample preparation method based on robot pipetting in the 96-well format we intended to then establish normal reference ranges for women and men, with saliva collected both in the early morning and late at night.

Method

Four hydrazides and one hydrazine were evaluated for their effectiveness as derivatization reagents based on the comparative signal response and efficacy of LC separation of five ketosteroid hydrazones via a methanol gradient mixed with e

SUBMITTER: Nadarajah N 

PROVIDER: S-EPMC11322771 | biostudies-literature | 2017 Apr-Aug

REPOSITORIES: biostudies-literature