Quantifying and Controlling the Proteolytic Degradation of Cell Adhesion Peptides.

ABSTRACT: Peptides are widely used within biomaterials to improve cell adhesion, incorporate bioactive ligands, and enable cell-mediated degradation of the matrix. While many of the peptides incorporated into biomaterials are intended to be present throughout the life of the material, their stability is not typically quantified during culture. In this work, we designed a series of peptide libraries containing four different N-terminal peptide functionalizations and three C-terminal functionalizations to better understand how simple modifications can be used to reduce the nonspecific degradation of peptides. We tested these libraries with three cell types commonly used in biomaterials research, including mesenchymal stem/stromal cells (hMSCs), endothelial cells, and macrophages, and quantified how th

SUBMITTER: Rozans SJ 

PROVIDER: S-EPMC11322908 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature