Minimal Peptide Sequences That Undergo Liquid-Liquid Phase Separation via Self-Coacervation or Complex Coacervation with ATP.

ABSTRACT: The simple (self-)coacervation of the minimal tryptophan/arginine peptide sequences W₂R₂ and W₃R₃ was observed in salt-free aqueous solution. The phase diagrams were mapped using turbidimetry and optical microscopy, and the coacervate droplets were imaged using confocal microscopy complemented by cryo-TEM to image smaller droplets. The droplet size distribution and stability were probed using dynamic light scattering, and the droplet surface potential was obtained from zeta potential measurements. SAXS was used to elucidate the structure within the coacervate droplets, and circular dichroism spectroscopy was used to probe the conformation of the peptides, a characteristic signature for cation-π interactions being present under conditions of coace

SUBMITTER: Castelletto V 

PROVIDER: S-EPMC11323023 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature