A mutation in Themis contributes to anaphylaxis severity following oral peanut challenge in CC027 mice.

ABSTRACT:

Background

The development of peanut allergy is due to a combination of genetic and environmental factors, although specific genes have proven difficult to identify. Previously, we reported that peanut-sensitized Collaborative Cross strain CC027/GeniUnc (CC027) mice develop anaphylaxis upon oral challenge to peanut, in contrast to C3H/HeJ (C3H) mice.

Objective

This study aimed to determine the genetic basis of orally induced anaphylaxis to peanut in CC027 mice.

Methods

A genetic mapping population between CC027 and C3H mice was designed to identify the genetic factors that drive oral anaphylaxis. A total of 356 CC027xC3H backcrossed mice were generated, sensitized to peanut, then challenged to peanut by oral gavage. Anaphylaxis and peanut-specific IgE were quantified

SUBMITTER: Risemberg EL 

PROVIDER: S-EPMC11323216 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature