Immunologic features of nontuberculous mycobacterial pulmonary disease based on spatially resolved whole transcriptomics.

ABSTRACT:

Background

The immunologic features of nontuberculous mycobacterial pulmonary disease (NTM-PD) are largely unclear. This study investigated the immunologic features of NTM-PD using digital spatial profiling techniques.

Methods

Lung tissues obtained from six patients with NTM-PD between January 1, 2006, and December 31, 2020, at Seoul National University Hospital were subjected to RNA sequencing. Cores from the peribronchial areas were stained with CD3, CD68, and DNASyto13, and gene expression at the whole-transcriptome level was quantified using PCR amplification and Illumina sequencing. Lung tissues from six patients with bronchiectasis collected during the same period were used as controls. The RNA sequencing results were validated using immunohistochemistry (IHC) in anoth

SUBMITTER: Koh J 

PROVIDER: S-EPMC11323347 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature