Dissecting reactive astrocyte responses: lineage tracing and morphology-based clustering.

ABSTRACT: Brain damage triggers diverse cellular and molecular events, with astrocytes playing a crucial role in activating local neuroprotective and reparative signaling within damaged neuronal circuits. Here, we investigated reactive astrocytes using a multidimensional approach to categorize their responses into different subtypes based on morphology. This approach utilized the StarTrack lineage tracer, single-cell imaging reconstruction and multivariate data analysis. Our findings identified three profiles of reactive astrocyte responses, categorized by their effects on cell size- and shape- related morphological parameters: "moderate", "strong," and "very strong". We also examined the heterogeneity of astrocyte reactivity, focusing on spatial and clonal distribution. Our research revealed a nota

SUBMITTER: Delgado-Garcia LM 

PROVIDER: S-EPMC11323641 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature