Activity-based protein profiling and global proteome analysis reveal MASTL as a potential therapeutic target in gastric cancer.

ABSTRACT:

Background

Gastric cancer (GC) is a prevalent malignancy with limited therapeutic options for advanced stages. This study aimed to identify novel therapeutic targets for GC by profiling HSP90 client kinases.

Methods

We used mass spectrometry-based activity-based protein profiling (ABPP) with a desthiobiotin-ATP probe, combined with sensitivity analysis of HSP90 inhibitors, to profile kinases in a panel of GC cell lines. We identified kinases regulated by HSP90 in inhibitor-sensitive cells and investigated the impact of MASTL knockdown on GC cell behavior. Global proteomic analysis following MASTL knockdown was performed, and bioinformatics tools were used to analyze the resulting data.

Results

Four kinases-MASTL, STK11, CHEK1, and MET-were identified as HSP90-regulate

SUBMITTER: Choi KM 

PROVIDER: S-EPMC11323684 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature