Pangenome evaluation of gene essentiality in Streptococcus pyogenes.

ABSTRACT: Bacterial species often consist of strains with variable gene content, collectively referred to as the pangenome. Variations in the genetic makeup of strains can alter bacterial physiology and fitness. To define biologically relevant genes of a genome, genome-wide transposon mutant libraries have been used to identify genes essential for survival or virulence in a given strain. Such phenotypic studies have been conducted in four different genotypes of the human pathogen Streptococcus pyogenes, yet challenges exist in comparing results across studies conducted in different genetic backgrounds and conditions. To advance genotype to phenotype inferences across different S. pyogenes strains, we built a pangenome database of 249 S. pyogenes reference genomes. We syst

SUBMITTER: Jespersen MG 

PROVIDER: S-EPMC11323703 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature