RECON gene disruption enhances host resistance to enable genome-wide evaluation of intracellular pathogen fitness during infection.

ABSTRACT: Transposon sequencing (Tn-seq) is a powerful genome-wide technique to assess bacterial fitness under varying growth conditions. However, screening via Tn-seq in vivo is challenging. Dose limitations and host restrictions create bottlenecks that diminish the transposon mutant pool being screened. Here, we have developed a murine model with a disruption in Akr1c13 that renders the resulting RECON^-/- mouse resistant to high-dose infection. We leveraged this model to perform a Tn-seq screen of the human pathogen Listeria monocytogenes in vivo. We identified 135 genes which were required for L. monocytogenes growth in mice including novel genes not previously identified for host survival. We identified organ-specific requirements for L. monocytogenes

SUBMITTER: Stamm CE 

PROVIDER: S-EPMC11323731 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature