Metabolic changes in <i>Toxoplasma gondii</i>-infected host cells measured by autofluorescence imaging.

ABSTRACT: Toxoplasma gondii, the causative agent of toxoplasmosis, is an obligate intracellular parasite that infects warm-blooded vertebrates across the world. In humans, seropositivity rates of T. gondii range from 10% to 90% across communities. Despite its prevalence, few studies address how T. gondii infection changes the metabolism of host cells. In this study, we investigate how T. gondii manipulates the host cell metabolic environment by monitoring the metabolic response over time using noninvasive autofluorescence lifetime imaging of single cells, metabolite analysis, extracellular flux analysis, and reactive oxygen species (ROS) production. Autofluorescence lifetime imaging indicates that infected host cells become more oxidized and have an increased proportion o

SUBMITTER: Gallego-Lopez GM 

PROVIDER: S-EPMC11323734 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature