Cationic-motif-modified exosomes for mRNA delivery to retinal photoreceptors.

ABSTRACT: Topical treatment of vitreoretinal diseases remains a challenge due to slow corneal uptake and systemic clearance. Exosomes are emerging nanocarriers for drug delivery due to biocompatibility and cellular targeting properties. To apply them for retinal targeting via the topical route, exosomes must traverse various ocular barriers including the cornea, lens, vitreous humor (VH), and the retina itself. Here we engineered high-purity milk-derived exosomes by anchoring arginine-rich cationic motifs via PEG₂₀₀₀ lipid insertion on their surface. Modification enabled exosomes to use weak-reversible electrostatic interactions with anionic glycosaminoglycan (GAG) and water content of the tissue to enhance their transport rate and retention. Addition of cationic motifs neut

SUBMITTER: Millan Cotto HA 

PROVIDER: S-EPMC11323772 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature