Activities and genetic interactions of fission yeast Aps1, a Nudix-type inositol pyrophosphatase and inorganic polyphosphatase.

ABSTRACT: Inositol pyrophosphate 1,5-IP₈ regulates expression of a fission yeast phosphate homeostasis regulon, comprising phosphate acquisition genes pho1, pho84, and tgp1, via its action as an agonist of precocious termination of transcription of the upstream lncRNAs that repress PHO mRNA synthesis. 1,5-IP₈ levels are dictated by a balance between the Asp1 N-terminal kinase domain that converts 5-IP₇ to 1,5-IP₈ and three inositol pyrophosphatases-the Asp1 C-terminal domain (a histidine acid phosphatase), Siw14 (a cysteinyl-phosphatase), and Aps1 (a Nudix enzyme). In this study, we report the biochemical and genetic characterization of Aps1 and an analysis of the effects of Asp1, Siw14, and Aps1 mutations on cellular inositol p

SUBMITTER: Ghosh S 

PROVIDER: S-EPMC11323792 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature