Cryo-EM architecture of a near-native stretch-sensitive membrane microdomain.

ABSTRACT: Biological membranes are partitioned into functional zones termed membrane microdomains, which contain specific lipids and proteins^1-3. The composition and organization of membrane microdomains remain controversial because few techniques are available that allow the visualization of lipids in situ without disrupting their native behaviour^3,4. The yeast eisosome, composed of the BAR-domain proteins Pil1 and Lsp1 (hereafter, Pil1/Lsp1), scaffolds a membrane compartment that senses and responds to mechanical stress by flattening and releasing sequestered factors^5-9. Here we isolated near-native eisosomes as helical tubules made up of a lattice of Pil1/Lsp1 bound to plasma membrane lipids, and solved their structures by helical reconstruction. Our structures re

SUBMITTER: Kefauver JM 

PROVIDER: S-EPMC11324527 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature