Structural dynamics of protein-protein association involved in the light-induced transition of Avena sativa LOV2 protein.

ABSTRACT: The Light-oxygen-voltage-sensing domain (LOV) superfamily, found in enzymes and signal transduction proteins, plays a crucial role in converting light signals into structural signals, mediating various biological mechanisms. While time-resolved spectroscopic studies have revealed the dynamics of the LOV-domain chromophore's electronic structures, understanding the structural changes in the protein moiety, particularly regarding light-induced dimerization, remains challenging. Here, we utilize time-resolved X-ray liquidography to capture the light-induced dimerization of Avena sativa LOV2. Our analysis unveils that dimerization occurs within milliseconds after the unfolding of the A'α and Jα helices in the microsecond time range. Notably, our findings suggest that protein-protein interactio

SUBMITTER: Kim C 

PROVIDER: S-EPMC11324726 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature