A multi-omics approach to elucidate okadaic acid-induced changes in human HepaRG hepatocarcinoma cells.

ABSTRACT: Okadaic acid (OA), a prevalent marine biotoxin found in shellfish, is known for causing acute gastrointestinal symptoms. Despite its potential to reach the bloodstream and the liver, the hepatic effects of OA are not well understood, highlighting a significant research gap. This study aims to comprehensively elucidate the impact of OA on the liver by examining the transcriptome, proteome, and phosphoproteome alterations in human HepaRG liver cells exposed to non-cytotoxic OA concentrations. We employed an integrative multi-omics approach, encompassing RNA sequencing, shotgun proteomics, phosphoproteomics, and targeted DigiWest analysis. This enabled a detailed exploration of gene and protein expression changes, alongside phosphorylation patterns under OA treatment. The study reveals concen

SUBMITTER: Wuerger LTD 

PROVIDER: S-EPMC11324782 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature