Development of a base editor for convenient and multiplex genome editing in cyanobacteria.

ABSTRACT: Cyanobacteria are important primary producers, contributing to 25% of the global carbon fixation through photosynthesis. They serve as model organisms to study the photosynthesis, and are important cell factories for synthetic biology. To enable efficient genetic dissection and metabolic engineering in cyanobacteria, effective and accurate genetic manipulation tools are required. However, genetic manipulation in cyanobacteria by the conventional homologous recombination-based method and the recently developed CRISPR-Cas gene editing system require complicated cloning steps, especially during multi-site editing and single base mutation. This restricts the extensive research on cyanobacteria and reduces its application potential. In this study, a highly efficient and convenient cytosine base

SUBMITTER: Li XD 

PROVIDER: S-EPMC11324792 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature