Generation of a conditional cellular senescence model using proximal tubule cells and fibroblasts from human kidneys.

ABSTRACT: Emerging evidence highlights cellular senescence's pivotal role in chronic kidney disease (CKD). Proximal tubule epithelial cells (PTECs) and fibroblasts are major players in CKD and serve as cellular sources of senescence. The generation of a conditionally immortalized human kidney cell model would allow to better understand the specific mechanisms and factors associated with cellular senescence in a controlled setting, devoid of potential confounding factors such as age and comorbidities. In addition, the availability of human kidney cell lines for preclinical research is sparse and most cell lines do not reflect their in vivo counterparts due to their altered behavior as immortalized cancer-like cells. In this study, PTECs and fibroblasts from human kidneys were isolated and transduced

SUBMITTER: Shao X 

PROVIDER: S-EPMC11324798 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature