Molecular basis of facilitated target search and sequence discrimination of TALE homeodomain transcription factor Meis1.

ABSTRACT: Transcription factors specifically bind to their consensus sequence motifs and regulate transcription efficiency. Transcription factors are also able to non-specifically contact the phosphate backbone of DNA through electrostatic interaction. The homeodomain of Meis1 TALE human transcription factor (Meis1-HD) recognizes its target DNA sequences via two DNA contact regions, the L1-α1 region and the α3 helix (specific binding mode). This study demonstrates that the non-specific binding mode of Meis1-HD is the energetically favored process during DNA binding, achieved by the interaction of the L1-α1 region with the phosphate backbone. An NMR dynamics study suggests that non-specific binding might set up an intermediate structure which can then rapidly and easily find the consensus region on a

SUBMITTER: Choi SR 

PROVIDER: S-EPMC11325038 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature