NCLX controls hepatic mitochondrial Ca²⁺ extrusion and couples hormone-mediated mitochondrial Ca²⁺ oscillations with gluconeogenesis.

ABSTRACT:

Objective

Hepatic Ca²⁺ signaling has been identified as a crucial key factor in driving gluconeogenesis. The involvement of mitochondria in hormone-induced Ca²⁺ signaling and their contribution to metabolic activity remain, however, poorly understood. Moreover, the molecular mechanism governing the mitochondrial Ca²⁺ efflux signaling remains unresolved. This study investigates the role of the Na⁺/Ca²⁺ exchanger, NCLX, in modulating hepatic mitochondrial Ca²⁺ efflux, and examines its physiological significance in hormonal hepatic Ca²⁺ signaling, gluconeogenesis, and mitochondrial bioenergetics.

Methods

Primary mouse hepatocytes from both an AAV-mediated conditional hepatic-specific and a total mitoch

SUBMITTER: Taha M 

PROVIDER: S-EPMC11325370 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature