Molecular cloning and sequence analysis of hamster CENP-A cDNA.

ABSTRACT:

Background

The centromere is a specialized locus that mediates chromosome movement during mitosis and meiosis. This chromosomal domain comprises a uniquely packaged form of heterochromatin that acts as a nucleus for the assembly of the kinetochore a trilaminar proteinaceous structure on the surface of each chromatid at the primary constriction. Kinetochores mediate interactions with the spindle fibers of the mitotic apparatus. Centromere protein A (CENP-A) is a histone H3-like protein specifically located to the inner plate of kinetochore at active centromeres. CENP-A works as a component of specialized nucleosomes at centromeres bound to arrays of repeat satellite DNA.

Results

We have cloned the hamster homologue of human and mouse CENP-A. The cDNA isolated was found to con

SUBMITTER: Figueroa J 

PROVIDER: S-EPMC113255 | biostudies-literature | 2002 May

REPOSITORIES: biostudies-literature