METTL3-mediated m⁶A modification of circGLIS3 promotes prostate cancer progression and represents a potential target for ARSI therapy.

ABSTRACT:

Background

Circular RNAs (circRNAs) have been shown to be involved in tumorigenesis and progression. However, the role of circGLIS3 (hsa_circ_0002874) in prostate cancer (PCa) has yet not been reported.

Methods

Candidate circRNA were determined through comprehensive analysis of public datasets, PCa cell lines, and tissues data. A series of cellular functional assays, including CCK-8, colony formation, wound healing, and transwell assays were performed. Subsequently, RNA sequencing, RNA immunoprecipitation, methylated RNA immunoprecipitation, microRNA pulldown, luciferase reporter assay, and western blot were used to explore the underlying molecular mechanisms. Moreover, the xenograft tumor mouse model was established to elucidate the function of circGLIS3.

Results

Cir

SUBMITTER: Cheng X 

PROVIDER: S-EPMC11325714 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature