Interaction networks within disease-associated Gα_S variants characterized by an integrative biophysical approach.

ABSTRACT: Activation of G proteins through nucleotide exchange initiates intracellular signaling cascades essential for life processes. Under normal conditions, nucleotide exchange is regulated by the formation of G protein-G protein-coupled receptor complexes. Single point mutations in the Gα subunit of G proteins bypass this interaction, leading to loss of function or constitutive gain of function, which is closely linked with the onset of multiple diseases. Despite the recognized significance of Gα mutations in disease pathology, structural information for most variants is lacking, potentially due to inherent protein dynamics that pose challenges for crystallography. To address this, we leveraged an integrative spectroscopic and computational approach to structurally characterize seven of the mos

SUBMITTER: Anazia K 

PROVIDER: S-EPMC11325797 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature