Tuning interdomain conjugation to enable in situ population modification in yeasts.

ABSTRACT: The ability to modify and control natural and engineered microbiomes is essential for biotechnology and biomedicine. Fungi are critical members of most microbiomes, yet technology for modifying the fungal members of a microbiome has lagged far behind that for bacteria. Interdomain conjugation (IDC) is a promising approach, as DNA transfer from bacterial cells to yeast enables in situ modification. While such genetic transfers have been known to naturally occur in a wide range of eukaryotes and are thought to contribute to their evolution, IDC has been understudied as a technique to control fungal or fungal-bacterial consortia. One major obstacle to the widespread use of IDC is its limited efficiency. In this work, we manipulated metabolic and physical interactions between geneticall

SUBMITTER: Stindt KR 

PROVIDER: S-EPMC11326116 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature