Integrative spatiotemporal modeling of biomolecular processes: application to the assembly of the Nuclear Pore Complex.

ABSTRACT: Dynamic processes involving biomolecules are essential for the function of the cell. Here, we introduce an integrative method for computing models of these processes based on multiple heterogeneous sources of information, including time-resolved experimental data and physical models of dynamic processes. We first compute integrative structure models at fixed time points and then optimally select and connect these snapshots into a series of trajectories that optimize the likelihood of both the snapshots and transitions between them. The method is demonstrated by application to the assembly process of the human Nuclear Pore Complex in the context of the reforming nuclear envelope during mitotic cell division, based on live-cell correlated electron tomography, bulk fluorescence correlation sp

SUBMITTER: Latham AP 

PROVIDER: S-EPMC11326192 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature