Optics-free Spatial Genomics for Mapping Mouse Brain Aging.

ABSTRACT: Spatial transcriptomics has revolutionized our understanding of cellular network dynamics in aging and disease by enabling the mapping of molecular and cellular organization across various anatomical locations. Despite these advances, current methods face significant challenges in throughput and cost, limiting their utility for comprehensive studies. To address these limitations, we introduce IRISeq (Imaging Reconstruction using Indexed Sequencing), a optics-free spatial transcriptomics platform that eliminates the need for predefined capture arrays or extensive imaging, allowing for the rapid and cost-effective processing of multiple tissue sections simultaneously. Its capacity to reconstruct images based solely on sequencing local DNA interactions allows for profiling of tissues w

SUBMITTER: Abdulraouf A 

PROVIDER: S-EPMC11326199 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature