Centrocortin potentiates co-translational localization of its mRNA to the centrosome via dynein.

ABSTRACT: Centrosomes rely upon proteins within the pericentriolar material to nucleate and organize microtubules. Several mRNAs also reside at centrosomes, although less is known about how and why they accumulate there. We previously showed that local Centrocortin (Cen) mRNA supports centrosome separation, microtubule organization, and viability in Drosophila embryos. Here, using Cen mRNA as a model, we examine mechanisms of centrosomal mRNA localization. We find that while the Cen N'-terminus is sufficient for protein enrichment at centrosomes, multiple domains cooperate to concentrate Cen mRNA at this location. We further identify an N'-terminal motif within Cen that is conserved among dynein cargo adaptor proteins and test its contribution to RNA localization.

SUBMITTER: Zein-Sabatto H 

PROVIDER: S-EPMC11326273 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature