Role of U11/U12 minor spliceosome gene <i>ZCRB1</i> in Ciliogenesis and WNT Signaling.

ABSTRACT: Despite the fact that 0.5% of human introns are processed by the U11/U12 minor spliceosome, the latter influences gene expression across multiple cellular processes. The ZCRB1 protein is a recently described core component of the U12 mono-snRNP minor spliceosome, but its functional significance to minor splicing, gene regulation, and biological signaling cascades is poorly understood. Using CRISPR-Cas9 and siRNA targeted knockout and knockdown strategies, we show that human cell lines with a partial reduction in ZCRB1 expression exhibit significant dysregulation of the splicing and expression of U12-type genes, primarily due to dysregulation of U12 mono-snRNA. RNA-Seq and targeted analyses of minor intron-containing genes indicate a downregulation in the expression of genes involved in cil

SUBMITTER: Powell-Rodgers G 

PROVIDER: S-EPMC11326282 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature