Characterisation of HBV and co-infection with HDV and HIV through spatial transcriptomics.

ABSTRACT:

Background and aims

The intrahepatic processes associated with chronic hepatitis B (CHB), especially in the context of hepatitis delta virus (HDV) and HIV co-infection, require a better understanding. Spatial transcriptomics can provide new insights into the complex intrahepatic biological processes, guiding new personalised treatments. Our aim is to evaluate this method characterising the intrahepatic transcriptional landscape, cellular composition and biological pathways in liver biopsy samples from patients with hepatitis B virus (HBV) and HDV or HIV co-infection.

Method

The NanoString GeoMx digital spatial profiling platform was employed to assess expression of HBV surface antigen and CD45 in formalin-fixed paraffin-embedded (FFPE) biopsies from three treatment-naïve pat

SUBMITTER: Cross A 

PROVIDER: S-EPMC11326438 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature