Monitoring GPCR conformation with GFP-inspired dyes.

ABSTRACT: Solvatochromic compounds have emerged as valuable environment-sensitive probes for biological research. Here we used thiol-reactive solvatochromic analogs of the green fluorescent protein (GFP) chromophore to track conformational changes in two proteins, recoverin and the A_2A adenosine receptor (A_2AAR). Two dyes showed Ca²⁺-induced fluorescence changes when attached to recoverin. Our best-performing dye, DyeC, exhibited agonist-induced changes in both intensity and shape of its fluorescence spectrum when attached to A_2AAR; none of these effects were observed with other common environment-sensitive dyes. Molecular dynamics simulations showed that activation of the A_2AAR led to a more confined and hydrophilic environment for DyeC. Additi

SUBMITTER: Belousov A 

PROVIDER: S-EPMC11326922 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature