Chinese medicine PaBing-II protects human iPSC-derived dopaminergic neurons from oxidative stress.

ABSTRACT:

Background

PaBing-II Formula (PB-II) is a traditional Chinese medicine for treating Parkinson's disease (PD). However, owing to the complexity of PB-II and the difficulty in obtaining human dopaminergic neurons (DAn), the mechanism of action of PB-II in PD treatment remains unclear. The aim of this study was to investigate the mechanisms underlying the therapeutic benefits of PB-II in patients with PD.

Methods

hiPSCs derived DAn were treated with H₂O₂ to construct the DAn oxidative damage model. SwissTargetPrediction was employed to predict the potential targets of the main compounds in serum after PB-II treatment. Metascape was used to analyze the pathways. Sprague-Dawley rats were used to construct the 6-hydroxydopamine (6-OHDA)-induced PD model, and

SUBMITTER: Wu S 

PROVIDER: S-EPMC11327085 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature