Analysis of CenKR essentiality in Sinorhizobium meliloti and its activity at a target gene promoter in vivo.

ABSTRACT: The two-component regulatory system CenK-CenR has recently emerged as a regulator of cell envelope and cell division processes in the alpha-proteobacteria. In Sinorhizobium meliloti, CenK-CenR regulates the expression of SrlA, a thioredoxin-domain protein of unknown function. Deletion of srlA causes sensitivity to salt and oxidizing agents on solid growth medium. In this work, we report that the response regulator CenR, but not the histidine kinase CenK, is essential for cell viability in S. meliloti. We also demonstrate that phosphorylation of the target residue D55 is not required for viability, suggesting that the unphosphorylated transcription factor sufficiently regulates expression of one or more essential genes in the genome. Using transcription assays and phenotype testing we exami

SUBMITTER: Freire IR 

PROVIDER: S-EPMC11328730 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature