Growth factor-dependent phosphorylation of Gα_i shapes canonical signaling by G protein-coupled receptors.

ABSTRACT: A long-standing question in the field of signal transduction is how distinct signaling pathways interact with each other to control cell behavior. Growth factor receptors and G protein-coupled receptors (GPCRs) are the two major signaling hubs in eukaryotes. Given that the mechanisms by which they signal independently have been extensively characterized, we investigated how they may cross-talk with each other. Using linear ion trap mass spectrometry and cell-based biophysical, biochemical, and phenotypic assays, we found at least three distinct ways in which epidermal growth factor affected canonical G protein signaling by the G_i-coupled GPCR CXCR4 through the phosphorylation of Gα_i. Phosphomimicking mutations in two residues in the α_E helix of Gα_i

SUBMITTER: Roy S 

PROVIDER: S-EPMC11328959 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature