Microheterogeneity in the Kinetics and Sex-Specific Response to Type I IFN.

ABSTRACT: The response to type I IFNs involves the rapid induction of prototypical IFN signature genes (ISGs). It is not known whether the tightly controlled ISG expression observed at the cell population level correctly represents the coherent responses of individual cells or whether it masks some heterogeneity in gene modules and/or responding cells. We performed a time-resolved single-cell analysis of the first 3 h after in vivo IFN stimulation in macrophages and CD4+ T and B lymphocytes from mice. All ISGs were generally induced in concert, with no clear cluster of faster- or slower-responding ISGs. Response kinetics differed between cell types: mostly homogeneous for macrophages, but with far more kinetic diversity among B and T lymphocytes, which included a distinct subset of nonresponsive cel

SUBMITTER: Gal-Oz ST 

PROVIDER: S-EPMC11328978 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature